Journal: Microbiology Spectrum

Article Title: Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii

doi: 10.1128/spectrum.04646-22

Figure Lengend Snippet: β-Lactamase transcript levels and MICs of A. baumannii IPTG-inducible β-lactamase strains and β-lactamase-overexpressing clinical isolates ^a

Article Snippet: Next, we measured the survival of Δ canB :: kanR in ATCC 17978 Tn 7 - bla OXA-23 and ATCC 17978 Tn 7 - bla TEM-1 strains with and without IPTG.

Techniques: Expressing

Journal: Microbiology Spectrum

Article Title: Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii

doi: 10.1128/spectrum.04646-22

Figure Lengend Snippet: Muropeptide composition of peptidoglycan isolated from the 17978 Tn 7 - bla ADC-7 strain (ADC) and the empty Tn 7 control strain (WT) ^a

Article Snippet: Next, we measured the survival of Δ canB :: kanR in ATCC 17978 Tn 7 - bla OXA-23 and ATCC 17978 Tn 7 - bla TEM-1 strains with and without IPTG.

Techniques: Isolation, Control, Concentration Assay

Journal: Microbiology Spectrum

Article Title: Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii

doi: 10.1128/spectrum.04646-22

Figure Lengend Snippet: A canB::EZ-Tn5kan insertion is conditionally essential during ADC-7 overexpression. Identification of canB :: EZ-Tn5kan as selectively lethal during ADC-7 overexpression. 17978 or 17978 Tn 7 - bla ADC-7 with canB :: EZ-Tn5kan transposon insertion was spotted on LB agar supplemented with 30 μg mL −1 kanamycin (control) and 5 mM IPTG to induce ADC-7 overexpression (IPTG).

Article Snippet: Next, we measured the survival of Δ canB :: kanR in ATCC 17978 Tn 7 - bla OXA-23 and ATCC 17978 Tn 7 - bla TEM-1 strains with and without IPTG.

Techniques: Over Expression, Control

Journal: Microbiology Spectrum

Article Title: Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii

doi: 10.1128/spectrum.04646-22

Figure Lengend Snippet: Percent survival of a Δ canB mutant under β-lactamase (ADC-7, OXA-23, TEM-1)-overexpressing conditions on plates supplemented with 5 mM NaHCO 3

Article Snippet: Next, we measured the survival of Δ canB :: kanR in ATCC 17978 Tn 7 - bla OXA-23 and ATCC 17978 Tn 7 - bla TEM-1 strains with and without IPTG.

Techniques: Mutagenesis

Journal: Microbiology Spectrum

Article Title: Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii

doi: 10.1128/spectrum.04646-22

Figure Lengend Snippet: Δ canB lethality is suppressed by mutations in A1S_2287 . The 17978 Δ canB :: kanR control strain and a strain containing an A1S_2287 ::T26 insertion were spotted on LB agar supplemented with increasing amounts of sodium bicarbonate (NaHCO 3 ).

Article Snippet: Next, we measured the survival of Δ canB :: kanR in ATCC 17978 Tn 7 - bla OXA-23 and ATCC 17978 Tn 7 - bla TEM-1 strains with and without IPTG.

Techniques: Control

Journal: Microorganisms

Article Title: Resistance and Co-Resistance of Metallo-Beta-Lactamase Genes in Diarrheal and Urinary-Tract Pathogens in Bangladesh

doi: 10.3390/microorganisms12081589

Figure Lengend Snippet: Phenotypic–genotypic association of β-lactamase genes and β-lactam antibiotic resistance.

Article Snippet: The bla TEM was found to coexist with bla OXA-type variants in 9.6% (5/52) of the MBL producers.

Techniques:

Journal: Veterinary and Animal Science

Article Title: Exploring of spectrum beta lactamase producing multidrug-resistant Salmonella enterica serovars in goat meat markets of Bangladesh

doi: 10.1016/j.vas.2024.100367

Figure Lengend Snippet: Primers used in PCR test of Salmonella enterica serovars and Beta-lactams resistant genes.

Article Snippet: This study specifically concentrated on the isolation and identification of ESBL-resistant genes ( bla TEM, bla SHV, bla CTX-M1, bla CTX-M2, bla CTX-M9, MultiCase ACC, MultiCase MOX, MultiCase DHA, bla OXA ) and the antibiogram profiling of Salmonella enterica serovars found in goat meat samples procured from retail outlets in Bangladesh.

Techniques: Sequencing

Journal: Veterinary and Animal Science

Article Title: Exploring of spectrum beta lactamase producing multidrug-resistant Salmonella enterica serovars in goat meat markets of Bangladesh

doi: 10.1016/j.vas.2024.100367

Figure Lengend Snippet: Amplified DNA of invA gene of Salmonella isolates at 284 bp, PC: Positive control as Salmonella spp. ATCC 700623 ( A); sefA gene of Salmonella Enteritidis Isolated at 488 bp, PC: Positive control as Salmonella enterica ser. Enteritidis ATCC 13076 ( B); fliC gene of Salmonella Typhimurium Isolated at 620 bp, PC: Positive control as Salmonella enterica ser. Typhimurium ATCC 700720 ( C); NC: Negative control (Nuclease free water) Amplified DNA of ESBL Resistance genes of Salmonella spp. from first multiplex PCR test. Lane M: 100bp DNA Ladder, Lane M: 100bp DNA ladder, bla TEM gene at 800bp position. Lane 2: bla SHV gene at 713 bp ( D).

Article Snippet: This study specifically concentrated on the isolation and identification of ESBL-resistant genes ( bla TEM, bla SHV, bla CTX-M1, bla CTX-M2, bla CTX-M9, MultiCase ACC, MultiCase MOX, MultiCase DHA, bla OXA ) and the antibiogram profiling of Salmonella enterica serovars found in goat meat samples procured from retail outlets in Bangladesh.

Techniques: Amplification, Positive Control, Isolation, Negative Control, Multiplex Assay

Journal: Veterinary and Animal Science

Article Title: Exploring of spectrum beta lactamase producing multidrug-resistant Salmonella enterica serovars in goat meat markets of Bangladesh

doi: 10.1016/j.vas.2024.100367

Figure Lengend Snippet: Frequency of beta-lactams resistance genes in Salmonella Enteritidis and Typhimurium isolated from retail goat meat.

Article Snippet: This study specifically concentrated on the isolation and identification of ESBL-resistant genes ( bla TEM, bla SHV, bla CTX-M1, bla CTX-M2, bla CTX-M9, MultiCase ACC, MultiCase MOX, MultiCase DHA, bla OXA ) and the antibiogram profiling of Salmonella enterica serovars found in goat meat samples procured from retail outlets in Bangladesh.

Techniques: Isolation

Journal: Microbiology Spectrum

Article Title: Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii

doi: 10.1128/spectrum.04646-22

Figure Lengend Snippet: β-Lactamase transcript levels and MICs of A. baumannii IPTG-inducible β-lactamase strains and β-lactamase-overexpressing clinical isolates ^a

Article Snippet: To incorporate the IPTG-inducible bla gene into the attTn7 locus within the ATCC 17978 chromosome, electrocompetent ATCC 17978 was coelectroporated with pTn 7 - bla ADC-7 or pTn 7 - bla TEM-1 , and the pTNS2 helper plasmid ( ) and transformants were selected on LB agar with apramycin, resulting in the strains ATCC 17978 Tn 7 - bla ADC-7 and ATCC 17978 Tn 7 - bla TEM-1 , respectively.

Techniques: Expressing

Journal: Microbiology Spectrum

Article Title: Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii

doi: 10.1128/spectrum.04646-22

Figure Lengend Snippet: Muropeptide composition of peptidoglycan isolated from the 17978 Tn 7 - bla ADC-7 strain (ADC) and the empty Tn 7 control strain (WT) ^a

Article Snippet: To incorporate the IPTG-inducible bla gene into the attTn7 locus within the ATCC 17978 chromosome, electrocompetent ATCC 17978 was coelectroporated with pTn 7 - bla ADC-7 or pTn 7 - bla TEM-1 , and the pTNS2 helper plasmid ( ) and transformants were selected on LB agar with apramycin, resulting in the strains ATCC 17978 Tn 7 - bla ADC-7 and ATCC 17978 Tn 7 - bla TEM-1 , respectively.

Techniques: Isolation, Control, Concentration Assay

Journal: Microbiology Spectrum

Article Title: Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii

doi: 10.1128/spectrum.04646-22

Figure Lengend Snippet: A canB::EZ-Tn5kan insertion is conditionally essential during ADC-7 overexpression. Identification of canB :: EZ-Tn5kan as selectively lethal during ADC-7 overexpression. 17978 or 17978 Tn 7 - bla ADC-7 with canB :: EZ-Tn5kan transposon insertion was spotted on LB agar supplemented with 30 μg mL −1 kanamycin (control) and 5 mM IPTG to induce ADC-7 overexpression (IPTG).

Article Snippet: To incorporate the IPTG-inducible bla gene into the attTn7 locus within the ATCC 17978 chromosome, electrocompetent ATCC 17978 was coelectroporated with pTn 7 - bla ADC-7 or pTn 7 - bla TEM-1 , and the pTNS2 helper plasmid ( ) and transformants were selected on LB agar with apramycin, resulting in the strains ATCC 17978 Tn 7 - bla ADC-7 and ATCC 17978 Tn 7 - bla TEM-1 , respectively.

Techniques: Over Expression, Control

Journal: Microbiology Spectrum

Article Title: Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii

doi: 10.1128/spectrum.04646-22

Figure Lengend Snippet: Percent survival of a Δ canB mutant under β-lactamase (ADC-7, OXA-23, TEM-1)-overexpressing conditions on plates supplemented with 5 mM NaHCO 3

Article Snippet: To incorporate the IPTG-inducible bla gene into the attTn7 locus within the ATCC 17978 chromosome, electrocompetent ATCC 17978 was coelectroporated with pTn 7 - bla ADC-7 or pTn 7 - bla TEM-1 , and the pTNS2 helper plasmid ( ) and transformants were selected on LB agar with apramycin, resulting in the strains ATCC 17978 Tn 7 - bla ADC-7 and ATCC 17978 Tn 7 - bla TEM-1 , respectively.

Techniques: Mutagenesis

Journal: Microbiology Spectrum

Article Title: Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii

doi: 10.1128/spectrum.04646-22

Figure Lengend Snippet: Δ canB lethality is suppressed by mutations in A1S_2287 . The 17978 Δ canB :: kanR control strain and a strain containing an A1S_2287 ::T26 insertion were spotted on LB agar supplemented with increasing amounts of sodium bicarbonate (NaHCO 3 ).

Article Snippet: To incorporate the IPTG-inducible bla gene into the attTn7 locus within the ATCC 17978 chromosome, electrocompetent ATCC 17978 was coelectroporated with pTn 7 - bla ADC-7 or pTn 7 - bla TEM-1 , and the pTNS2 helper plasmid ( ) and transformants were selected on LB agar with apramycin, resulting in the strains ATCC 17978 Tn 7 - bla ADC-7 and ATCC 17978 Tn 7 - bla TEM-1 , respectively.

Techniques: Control